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Silica gel 60 F254 (Precoated aluminium plates) from Merck was used to monitor reaction progress.
NMR was used to monitor reaction progress.
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Thin layer chromatography (Merck silica gel G) was used to monitor the reaction progress.
FT-IR and SEM-EDX techniques were employed to monitor the reaction progress.
The UV-visible absorption spectra of the filtrates were recorded at room temperature to monitor the reaction progress.
Fourier transform infrared spectroscopy (FTIR) was used to monitor the reaction progress in each step of the syntheses.
To improve this situation, we want to use the TOF mass spectrometer to monitor the reaction progress by ejecting the Coulomb crystal after a certain time.
An alternative way to monitor the reaction progress through simple image analysis of the H2 bubble shrinkage is also studied and discussed.
These results indicate that the enrichment factor provides a powerful tool to monitor the reaction progress, and thus the performance, of an iron-reactive barrier over time.
Scanning electron microscopy was used to ensure that the porous structures of the scaffolds were preserved throughout the multiple conjugation steps, and Fourier transform infrared spectroscopy was used to monitor the reaction progress.
Formation of the internal disulphide bond was achieved by overnight stirring in an aqueous redox buffer (150 mM Tris-HCl pH 8.0, 1 mM EDTA, 10 mM reduced glutathione, 1 mM oxidised glutathione) while monitoring the reaction progress by MALDI-TOF mass spectrometry.
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