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We used light transmission aggregometry (LTA) the gold standard method to monitor platelet function inhibition [11].
To monitor platelet function in patients with myocardial infarction (n=46) who were treated with the fibrinolytic agent reteplase, the glycoprotein (GP) IIb/IIIa blocker abciximab, and the ADP receptor antagonist ticlopidine, we developed a flow cytometric assay.Binding of abciximab to platelets was directly monitored as the percentage of platelets stained by a goat anti-mouse antibody.
Impedance aggregometry is used to monitor platelet function in patients with inherited platelet dysfunctions as well as aspirin®- and clopidogrel therapy to detect non-responders or anti-platelet drug resistance respectively [ 14- 16].
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Platelet inhibition that was achieved during the first 24 hours by abciximab was directly maintained by additional treatment with ticlopidine.Flow cytometric monitoring of platelet function allows differentiation of the effects of reteplase, abciximab, and ticlopidine.
The use of clopidogrel (Plavix), an inhibitor of adenosine diphosphate (ADP -induced plADP -inducedgation, has been plateleto reduce ischemic events in caggregationar patients, but little information is available for optimal monitoring of platelet function in patients receiving thasdrug preoperatively.
These analyzers monitor different aspects of platelet function and appear to be technically reliable and practical POC devices, despite limitations [ 65, 80- 82].
However, in the absence of a large-scale prospective clinical trial, routine platelet function monitoring and modification of timing of surgery based on platelet function monitoring are currently not recommended.
Platelet function monitoring in patients with coronary artery disease.
Clopidogrel (Plavix) and cardiac surgical patients: implications for platelet function monitoring and postoperative bleeding.
When nursing on valproate can not be avoided, monitoring for liver and platelet function in the child is advised [59, 61, 64].
Currently, there is limited evidence of an association between platelet inhibition and coronary artery bypass grafting (CABG)- related bleeding in patients on clopidogrel therapy indicating that preoperative platelet function monitoring may guide both the timing of elective CABG and the administration of blood products in patients needing surgery.
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