In order to account for undifferentiated stem cells with high C=C saturation, the fabricated structure was merged with SERS, which was highly effective in monitoring neural stem cell differentiation.
In this study, we investigated the survival and proliferation of injectable hydrogel-encapsulated stem cells by non-invasively monitoring human neural stem cells carrying the highly sensitive luciferase gene.
Diagnostic imaging serves to precisely monitor disease onset and progression while neural stem cell replacement can regenerate damaged tissue through control of stem cell fates.
DCs transduced with antigens from CSCs might elicit immune responses against normal stem cells; therefore, to monitor for possible cross-reaction against neural stem cells, we performed regular ophthalmologic exams to identify the development of inflammatory reactions against such cells in the eye [ 46].
This work establishes the efficacy of in vivo monitoring of the viability of implanted neural stem cells, incorporated within different types of bioactive hydrogel.
We examined the in vivo effectiveness of different elastic types of hydrogels encapsulating viable neural stem cells by successfully monitoring the proliferation of implanted stem cells incorporated within a 3D hydrogel scaffold.
To monitor for a possible cross-reaction against somatic neural stem cells, the patients underwent serial ophthalmological evaluations, and none of the patients developed retinal or uveal inflammation.
Single adult neural stem cells were seeded followed by monitoring over a period of four days, resulting in spheres in the range of 8 64 cells (Fig. 4).
Additionally, the CC is located adjacent to the subventricular zone, a potential source for neural stem cells, making the CC ideally located to monitor neuroregenerative responses for repair of demyelination.
Here, we describe the safety and functional outcome monitoring results for the final trial cohort, consisting of 6 ALS subjects receiving 5 unilateral cervical intraspinal neural stem cell injections.
