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It also demonstrated that MitoNuc1 in living cells can monitor mitochondrial division in real time and revealed nucleolar ultrastructure under stimulated emission depletion nanoscopy.
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At the same time, it supports mitochondrial division and integrates mitochondrial dynamics into cellular architecture.
JC-1 staining was used to monitor mitochondrial membrane potential and mitochondrial injury.
Itoh, K., Tamura, Y., Iijima, M. & Sesaki, H. Effects of Fcj1-Mos1 and mitochondrial division on aggregation of mitochondrial DNA nucleoids and organelle morphology.
Mitochondrial division inhibitor (mdivi-1), a selective inhibitor of a mitochondrial fission protein dynamin-related protein 1 (Drp1), has been shown to exert protective effects in heart and cerebral ischemia reperfusion models.
In order to monitor mitochondrial Ca2+ levels, we transfected cells with a mitochondrial targeted Ca2+ indicator, Mito-GCaMP3.
Conventional TEM has also some technical limitations in verifying mitochondrial division.
As is well known, the resolution of fluorescence microscopy(~200 nm) is insufficient to distinguish structural details such as double mitochondrial membrane in mitochondrial division.
Mitochondrial division is important for mitochondrial distribution and function.
ERMES marks sites of ER-associated mitochondrial division.
10.7554/eLife.00422.015 Figure 4. Nucleoid segregation is linked to mitochondrial division.
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