Next, we monitored its expression using an RSL2 GFP reporter line.
To address whether higher pH in 3d/3d BM-DCss was dependent on NOX2, we monitored its expression and activity in phagosomes30.
To determine in which tissue(s) SMN is expressed in Drosophila we raised antibodies against full-length Drosophila SMN (See Materials and Methods) and monitored its expression pattern particularly at the NMJ.
The fact that HB-19 forms a stable complex with the cell-surface expressed nucleolin, incubation of cells with biotinylated HB-19 (HB-19/Btn) followed by purification of surface/cytoplasmic extracts using avidin-agarose provides an efficient method for purification surface nucleolin and monitoring its expression [8].
Since Pst-AvrRpm1 is able to induce the P5CS2 gene expression at advanced HR stages (since 24 hpi, long-day grown plants; Ref. 8), we monitored its expression in our samples.
But TMO7=PRE3 and this gene is expressed in young leaves (see Author response image 2 from the Bellini lab), so we would not be able to conclude from monitoring its expression whether cells had an early epidermal or meristem identity.
Given that monocytes are the main TLR-expressing cell type in leukocytes 25, the main target for C5a in PBMCs, and that they orchestrate many of the TLR-induced responses of peripheral blood leukocytes, including those of neutrophils 25, we focused on monocyte C5aR and monitored its expression in PBMCs after a 30-min pre-exposure to LPS.
To gain further insights on the role of CR1 in CSCs, we monitored its expression in colon tumor spheroids over time and we found a marked oscillation of CR1 surface levels, which fluctuated between ~1 and 60% with a rough sinusoidal distribution.
Although the EGFP-tagged gene served as a useful reporter system to monitor its tissue specific gene expression in living animals, growing evidences suggested that EGFP itself can alter important cellular functions such as protein stability29.
To further define the function of JunD, we monitored its pattern of expression in ras-derived tumours.
