After the induction of a VSG synthesis block, the decrease in incorporation of [3H]-serine into protein resembled that seen monitoring incorporation of [35S]-methionine.
All methylation assays monitored incorporation of tritiated methyl groups in to DNA by using a modified ion-exchange filter binding assay [23].
The assay consists of membranes from Hhat transfected cells, purified recombinant Shh protein, and 125I-Iodopalmitoyl CoA, and monitors incorporation of 125I-Iodopalmitate into Shh (11).
The inability of any one amino acid (i.e. Ser, Cys, or Thr) to disproportionately influence apparent missense incorporation suggests that the assay monitored incorporation errors rather than altered luciferase activity arising from incorporation of any specific amino acid.
We monitored incorporation of k6W-Ub into Ub-protein conjugates, deubiquitination, protein aggregation, cell proliferation, and differentiation, including lens-specific-crystallin expression, proper fiber formation, denucleation and lens clarity.
Thus, monitoring incorporation of n-3 FA into EM is a suitable model for monitoring the FA intake[ 17].
Inactivation of TBL1 by siRNA delivery into Capan-1 cells reduced the cellular glucose uptake by 30% as measured 48 h after transfection by monitoring incorporation of radio-labeled 2-deoxyglucose over 15 min (Fig 2C).
To do this, we assayed macromolecular synthesis by bacterial cells in culture, monitoring incorporation of [C]-thymidine into DNA, [C]-uracil into RNA, and [C]-amino acids into protein.
Beyond the detectability, the earliest response time and spatial coverage constraints of a monitoring network design are considered, and the role of adaptive monitoring strategies for compliance monitoring and incorporation of expert judgment on likely leakage locations is considered.
An analogous approach is to use mass spectrometry to monitor the incorporation or release of stable isotopes as substrate is converted into product.
