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Thus, methods to noninvasively monitor gene transfer using SSTR2-based reporters may have wide applicability towards improving and assessing gene therapy; further example applications may include vector development, assessing promoter activity, and designing dosing regimens.
The imaging of gene expression is critical to monitor gene transfer.
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Lentiviral vectors are used extensively in therapeutic gene transfer, so monitoring for related events of proliferation-promoting integration with these vectors during gene therapy is important.
The observed high rate of HGT makes rumen Ciliates ideal bio-monitors for identification of potential gene transfer events in the gastro-intestinal tract of farm animals.
We finally monitored potential deleterious effects of rAAV gene transfer on the hypertrophic and terminal differentiation in bone marrow aspirates maintained over time in dynamic and static culture conditions while testing the influence of the candidate sox9 gene transfer on such processes using this vector class.
To further verify gene transfer, GFP and GUS expression were monitored on the whole plant level.
Gene transfer between different species is generally termed horizontal gene transfer (HGT) or lateral gene transfer (LGT).
Next we evaluated whether the expression of the Bax Bax and the Bax Cyt c split YFP pairs by hepatocellular somatic gene transfer (using hydrodynamic DNA injection) can be used to monitor apoptosis induced by anti-Fas antibody in liver hepatocytes.
These two vectors were also used to check where gene transfer was accomplished by Agrobacterium on cotyledonary node explants monitoring (transient) gene expression of GUS and DsRed, two days after cocultivation.
The hSSTr2 was monitored as a reporter gene for 99mTc-P2045 (an SST analogue) imaging showed adenoviral gene transfer to cancers, such as ovarian cancer [ 85].
These in vivo hSSTr2 based imaging methods have several advantages: repetitively observing the expression levels of hSSTr2 or other genes within transfected tumors [ 30], studying the biodistribution of gene transfer vectors [ 25], predicting the effects of PRRT, and monitoring the time course of tumor growth and the efficacy of antitumor gene therapy [ 21].
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