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This information allowed the development of microarrays able to monitor gene expression changes at the genome scale.
In this study we used microarrays to monitor gene expression changes in response to VPA during stages critical to VPA-induced NTDs in the mouse embryo.
This dataset can be interrogated to monitor gene expression changes occurring during the immediate transition from in vitro to in vivo growth.
To monitor gene expression changes associated with VPA teratogenicity, we adopted conditions of early exposure previously reported to induce NTDs in approximately 60% of live fetuses in the NMRI strain, as observed 18 dpc (Nau and Löscher 1986).
Quantitative real-time PCR was used as the approach to monitor gene expression changes, and MdHsf transcript abundance in developing flowers/fruits was compared to that of vegetative leaf tissue (3 5 cm in length).
To monitor gene expression changes in response to OLE1 or RSP5 depletion, strains SN95 (CaLC2993), CaLC3032 (tetO-OLE1/ole1Δ), and CaLC3366 tetO-RSP5/rsp5Δ tetO-RSP5/rsp5Δernight at 30°C in YPD, were shakingrown200 rpm.
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For instance, quantification of mRNA abundance is an effective way for monitoring gene expression changes in response to the drugs.
Monitoring gene expression changes during commitment again indicated that commitment was mediated by stochastic and independent modulation of key regulators.
Blood has been shown to be a responsive tissue that is useful for monitoring gene expression changes due to disease, environmental, biological or drug effects.
The Bovine Innate Immune Microarray will be useful for monitoring gene expression changes as a response to infection in a wide range of bovine and ovine tissues.
Interestingly, although we monitored gene expression changes in relation to growth rate in carbon-limited conditions, also nitrogen-responsive regulators and their corresponding transcription targets are downregulated with increasing growth rate (see Supporting information, Data S1).
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