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CAGE has previously been used to define global transcription start site usage and monitor gene activity in mammals.
For instance, 'reporter genes' which are part of a gene therapy can be used to determine if transfection was successful and to monitor gene activity and distribution in the individual patient [13].
Now, with more ways to monitor gene activity during development—and fully sequenced genomes of various snakes and other reptiles for comparison Cohn and graduate student Francisca Leal have tracked the genetic activity in embryonic pythons to see why their legs start, but never finish, developing.
In this study we used GFP fusions, rather than transcript profiling, to monitor gene activity (Barelle et al., 2004).
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To investigate possible effects of salinity on CNGC19 and CNGC20, we monitored gene activities using reporter genes and quantitative RT-PCR.
To regulate the Tet-responder genes, we employed transgenic mice with both forebrain-specific tTA [9] and rtTA expression (present study) and monitored responder gene activity by enzymatic activity and immunohistological analyses of brain slices.
A luminescence detection system is described that couples a genetically engineered bioluminescent reporter organism and fiber optic technology for monitoring in situ reporter gene activity in porous media under dynamic conditions.
Young plasma jabs ramped the gene activity back up again.
The activity also turns on DNA fragments that Cambia takes from yeast that amplify gene activity.
Thus, methods to noninvasively monitor gene transfer using SSTR2-based reporters may have wide applicability towards improving and assessing gene therapy; further example applications may include vector development, assessing promoter activity, and designing dosing regimens.
A new study indicates that poor sleep can significantly disrupt and inhibit normal gene activity in hundreds of genes.
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