Sentence examples for monitor for transfection from inspiring English sources

To monitor for transfection efficiency, we co-expressed pEGFP-N2 (Clontech) in a number of experiments.

Cells were cotransfected with the reporter plasmid containing the promoter of VEGF (-2362 to +90 nt sequence of human Vegf gene) and a pRL-TK plasmid (as a monitor for transfection efficiency).

An aliquot of the transfected cells was routinely monitored for transfection efficiency by immunofluorescence assay and Western blot analysis with anti-Flag antibodies.

Expression of truncated green fluorescence protein (tGFP) and a puromycin resistance cassette allow for monitor transfection and selection of transfected transgenic cells.

Cells were transfected with an equimolar amount of a given Plp1-lacZ construct and a fixed amount (0.35 μg/well) of a plasmid [RSVL (Rous sarcoma virus-luciferase)] containing the luciferase reporter gene under control of the LTR (long terminal repeat) promoter of RSV (rous sarcoma virus) to monitor for differences in transfection efficiency.

However, readthrough assays based on single reporter genes lack an internal control for initiating ribosomes and in consequence require careful monitoring of transfection efficiencies, reporter gene mRNA levels and translational efficiency between experiments.

After 24 h, transiently transfected cells were checked for transfection efficiency by monitoring GFP expression under fluorescence microscope and were used for experiments.

A FAM-labeled, random sequence pre-miR was used for monitoring transfection efficiency by flow cytometry.

Plasmid Renilla luciferase (PRLSV40) was used as internal control for monitoring transfection efficiency.

pCMVβ-galactosidase plasmid (Clontech, Palo Alto, CA, USA) was used for monitoring transfection efficiency.

For monitoring transfection efficiency pSV40-renillaLuc was cotransfected.