This approach involves monitoring for endogenous changes (an effect) in the body [e.g., by using molecular profiling to note changes in messenger RNA (transcriptomics), proteins (proteinomics), and endogenous metabolites (metabolomics)] (Wilson and Suk 2003).
Peptides were eluted using a 75-min gradient with solvent A (1% acetic acid) and B (water/acetonitrile/acetic acid at 10 89 1, by vol).: 10 min from 0 to 10% B, 45 min from 10 to 34% B, 10 min from 34 to 47% B, and 10 min from 47 to 100% B. Transitions were monitored for endogenous and heavy phosphopeptides as detailed in Supplementary Table S3 (at http://www.biochemj.org/bj/454/bj4540501add.htm).htm
The sensing test paper is simple, rapid and inexpensive, and serves as a visual platform for ultrasensitive monitoring of endogenous Cu2+ in human urine.
A variety of the catalase-based biosensor designs described here show promise for in vivo monitoring of endogenous H2O2 in the brain.
Recent discoveries of different modes of exocytosis and a plethora of molecules involved in neurotransmitter release has resulted in demand for more rapid and efficient methods for monitoring endogenous glutamate release from various tissue sources.
Toward this goal, we have rationally designed and synthesized a novel two-photon fluorescence probe FC-βgal for monitoring endogenous β-gal in lysosome.
Notably, DFB1 manifests significantly dual-channel and turn-on NIR fluorescent signals simultaneously in response to Cys concentration, which make it favorable for monitoring endogenous Cys activity in vivo.
The WFMP-FLIM method might be used to disclose correlations between molecular interactions and metabolic changes since PIE is extensible to a third pulsed laser source for monitoring endogenous fluorescence of living cells (NAD(P H or flavin cofactors as FMN and FAD).
We here designed a NIR fluorescent probe for monitoring the endogenous production of H2S in living cells.
Because low resistance seals (<20 MΩ) do not influence the cell membrane and because the normal intracellular milieu is maintained, this approach is the least invasive method <span class="lh">for monitoring the endogenous electrical activity of single cells.
