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However, specialized instruments designed to monitor fluorescence changes during heating are required.
An iXon+ 885 charge-coupled device camera (Andor Technology) was used to monitor fluorescence changes.
Series of 500 frames sampled at 98 ms per frame (128 × 128 pixels per frame) were used initially to monitor fluorescence changes over time.
Series of 500 frames (128 × 64 pixels per frame) were collected at a scanning frequency of 25 ms per frame to monitor fluorescence changes over time.
To investigate the possibility that 1,2-NQ generates H2O2 extracellularly, we used a plate reader assay to monitor fluorescence changes in BEAS-2B cells expressing roGFP-cyto or HyPer-cyto with various 1,2-NQ concentrations (10 150 µM) in the presence or absence of exogenous catalase.
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We used optimized multiphoton imaging conditions that do not damage homozygous pde6cw59 cones to monitor GCaMP3 fluorescence changes in mutant cells that degenerate during the 9 h time-lapse.
The internalization efficacy and bioavailability of encapsulated DQ-ovalbumin were assessed by monitoring the fluorescence changes in the cargo.
To confirm the rapid response of BSA-GNPs to Hg2+, we measured the response time of fluorescence quenching by monitoring the fluorescence changes for 5 min after the addition of Hg2+ into the BSA-GNPs (Fig. S4).
By monitoring the fluorescence changes arose from different interactions of Sybr Green I with Cu100 and Cu100/Cu2+ complex, the Cu2+ could be linearly detected from 5.57 to 250 ppb, with a detection limit of 5.57 ppb.
The present work deals with interplay of proflavine (PF) in between the nano-cavity of a supramolecular host, cucurbit[7]uril (CB7) and DNA by monitoring the fluorescence changes of proflavine using steady-state and picosecond time-resolved spectroscopic techniques.
This was followed by warming the cells to 37°C and then monitoring the fluorescence changes in the cell sample using a fluorescence spectrophotometer.
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Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com