Exact(8)
Surrogate standards were added to each sample to monitor extraction performance and matrix effects.
An internal control is needed to monitor extraction efficiency and to detect inhibition.
The results of this particular application of implementing NIR spectroscopy to monitor extraction process are very encouraging.
An internal control consisting of phocine herpes virus was processed along with the specimens during the entire procedure and permitted to monitor extraction and amplification efficiency, including inhibition.
All samples were processed in the presence of internal standards to monitor extraction efficiency and verify measurement accuracy.
A human RNaseP gene PCR was used as an internal control to confirm the absence of PCR inhibition and to monitor extraction efficiency.
Similar(52)
Monitoring extraction of these seven endogenous and three spike-in sequences extracted from 10 separate aliquots of the pooled plasma sample showed increased RNA yields from the QIAGEN RNeasy and miRNeasy kits compared to the other three protocols tested (Table 2).
The set-up is fully automated and features monitored extraction, which facilitates rapid optimization of the method.
As a result, the application of on-line NIR spectroscopy for monitoring extraction process in CHM could be very encouraging and reliable.
To monitor the extraction efficiency, linearity of the extraction yield, presence of co-purified inhibitors and bias associated with fragment size, we developed an in-house artificial spike-in material containing small (approximately 100 bp), medium (approximately 500 bp) and large (approximately 1,500 bp) fragment sizes.
A complete central composite 2³ factorial experimental design has been used to monitor the extraction characteristics, as affected by different variables, extraction time (ET), liquid-to-solid ratio (LSR), and microwave power (MWP).
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