Therefore, the hallmark of our technique is its capacity to monitor excessive NOX enzyme function as a reliable indicator of oxidative stress, rather than dissecting the activation of specific NOX isotypes.
The importance of electrostatic interactions for enzyme function necessitates methodologies that are capable of monitoring local changes in electrostatics in conjunction with catalysis.
These adducts were shown to be useful substrates for polyP-degrading enzymes, allowing us to monitor enzyme activity spectrophotometrically in real time; furthermore, we used these substrates to identify a new function for the clinically significant enzyme, Nudt2.
The ability to impartially monitor metabolic transformations from a variety of biological sources has also allowed metabolomics to thrive within the context of enzyme function discovery, typically in combination with recombinant genetic and protein expression tools.
Gerlt, J.A. et al. The Enzyme Function Initiative.
Neet, Cooperactivity in Enzyme Function: Equilibrium and Kinetic Aspects.
Albery, W. J. & Knowles, J. R. Evolution of enzyme function and the development of catalytic efficiency.
An additional set of targets was announced consisting of 1,301 enzymes from multiple species and metagenomic studies that were the focus of the Enzyme Function Initiative project51.
EC-BLAST has the potential to improve enzyme classification, identify previously uncharacterized or new biochemical transformations, improve the assignment of enzyme function to sequences, and assist in enzyme engineering.
These readily reproducible methods can be used to improve enzyme function by directed evolution, to covalently immobilize enzymes, to microencapsulate enzymes and cells, and to manufacture enzymes for human health, nutrition, and environmental protection.
