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Multifunctional porous Si nanostructure is designed to optically monitor enzymatic activity of horseradish peroxidase.
Two independent assays, using either 3-OH decanoyl-NAC or acetoacetyl-coenzyme A (AcAcCoA) as substrate, were established to monitor enzymatic activity of FabG.
Beyond cell tracking, MRI now has the added potential of being used to monitor enzymatic activity, gene expression, metal ion homeostasis and cell death in vivo.
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Next, we investigated the potential of this platform to act as an optical biosensor for monitoring enzymatic activity through RIFTS experiments.
They have demonstrated that the designed GOD-coated SWNTs are capable of monitoring enzymatic activity at the single molecule level of an individual SWNT [77].
Monitoring enzymatic activity in vivo of individual homologous enzymes such as the matrix metalloproteinases (MMPs) by antagonist molecules is highly desired for defining physiological and pathophysiological pathways.
A liquid crystal (LC -based sensor, which is capabLC -baseditoring enzymatic activity at the aqueousensornterface and detecting cellulase and cysteine (Cys), whichereis reported.
Monitoring enzymatic activities at the cell surface is challenging due to the poor efficiency of transport and membrane integration of fluorescence resonance energy transfer (FRET -based biosensors.
To estimate timeframe for cultivation prior to RNA isolation, we monitored enzymatic activities of PFOR and ME (Rasoloson et al. 2002) as their expression is known to be affected by iron availability in trichomonads (Vanácová et al. 2001).
To monitor the enzymatic activity, the reducing sugars released by the action of enzymes are determined colorimetrically.
These collagen fragments specifically monitor the enzymatic activity of cathepsin K [ 55] and do not reveal other compensatory organic resorption processes or the continued resorption of the inorganic matrix.
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