Sentence examples for monitor conformational changes from inspiring English sources
Importantly, the authors confirmed these computational findings directly in biophysical studies, using arrestin mutants tagged with fluorescent labels to monitor conformational changes at the protein surfaces that interact with the receptor's core and C terminus.
To address this we have used chemical nucleases tethered to the polymerase to monitor conformational changes, and engineered disulfide bonds to block conformational changes at defined steps in transcription.
These limitations suggest that new tagging processes, permitting the location of the right fluorescent markers at the right places, must be found to built up inter- and/or intra-molecular rulers allowing one to monitor conformational changes resulting from intracellular protein-protein, protein-membrane, and enzyme-substrate binding.
This labeling technique allows for the elimination of over-labeling the protein and is necessary when using FRET to monitor conformational changes [9].
Accordingly, they monitor conformational changes in the transporter in real time.
The tryptophan fluorescence can be used to monitor conformational changes in native protein and protein protein interactions.
Besides monitoring conformational changes within the unfoldase, these measurements will also help to follow the path of substrate molecules during initial binding, engagement, unfolding, and translocation.
We first monitored conformational changes in Bax as indicators of its activation.
In conclusion we have developed a robust and efficient F NMR method for monitoring conformational changes and specific ligand binding to NDM-1.
We subsequently monitored conformational changes by HSQC spectroscopy upon titrating the domain with 14 salts: namely Na 2SO 4, NaF, NaSCN, Na 2HPO 4, NaCl, NaBr, NaNO 3, NaI, MgCl 2, KCl, CaCl 2, guanidinium chloride (GdmCl), LiF, and KCl.
To monitor the conformational changes during the simulations, the root mean squared deviation (RMSD) between the initial coordinates of the backbone atoms of the proteins and the coordinates along the MD simulations was computed for every snapshot.
