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The results also serve as a reference for further studies designed to detect or monitor cone photoreceptors in patients with retinal diseases.
The clinical applications of such measurements could be substantial; with the ability to monitor cone structure and function, researchers would be positioned to elucidate more clearly the disease sequence of retinal degenerations, and also provide additional tools for assessing therapeutic efficacy in individuals receiving intervention.
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As supportive evidence of cone visualization and monitoring, cone spacing as well as outer segment (OS) lengths were measured (in some cases repeatedly) and compared to the literature.
Multiple reaction monitoring transitions were monitored; the cone voltages and collision energies used were as follows: 8‐oxo‐dG, [284→168, 35 V, 14 eV], and N5‐8‐oxodG, [289→173, 35 V, 14 eV].
Subsequent experiments required the use of a higher magnification (100×) objective to monitor growth cone behaviour.
Historically, groundwater level monitoring wells in Beijing Plain, China, were installed for assessing groundwater resources and for monitoring the cone of depression.
The purpose of this study is to demonstrate the feasibility of cone monitoring using ultrahigh-resolution adaptive optics optical coherence tomography.
The variation in respiratory activity during NGF-induced axonal growth was monitored at the growth cone of the axon.
For D933-mer, the transitions monitored were m/z 981.04+→263.1+ (30V cone voltage, 27eV collision energy).
For unlabeled 33-mer internal standard, the transitions monitored were m/z 978.84+→263.1+ (30V cone voltage, 27eV collision energy) for the quantitation assay and m/z 1304.73+→263.1+ (40V cone voltage, 50eV collision energy) as a confirmatory transition.
We know of no other imaging modality that can monitor foveal or parafoveal cones over time with comparable resolution in all three dimensions.
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