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A constitutive Renilla luciferase vector was included in all mixes (40 1) to normalize transfection efficiency and monitor cell viability.
Resazurin-based redox metabolic assays have been used to monitor cell viability in both two- and three-dimensional cell cultures.
The bioelectrochemical assay was optimized to obtain high sensitivity and the method was applied to monitor cell viability and screen susceptibility of metabolically active cells (E. coli, B. subtilis, Enterococcus, P. aeruginosa and Salmonella typhi) to antibiotics such as ampicillin and kanamycin.
Cell proliferation reagent WST-1 (Roche) was used to monitor cell viability according to the manufacturer's instructions.
YO-PRO-1 nucleic acid staining (Invitrogen) was used to monitor cell viability by incubating with cells for 20 min on ice.
They were counterstained with 2 µM TOPRO-3 (1 mg/ml stock solution) to monitor cell viability just before flow cytometry analysis.
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Here we developed a method for monitoring cell viability and proliferation in bio-engineered organ constructs using a resazurin perfusion assay.
As LPS is a known inducer of TNFα in macrophages, we treated iPS-MΦ with 5 µg/mL TNFα neutralizing antibody and stimulated them with 10 ng/mL LPS and monitored cell viability by video microscopy over 12 h (Supplementary Figure 6C).
Antioxidant properties against cytotoxic effect of H2O2 were evaluated by monitoring cell viability.
In these reports, they monitored cell viability and anticancer drug activities of the cell suspension and immobilized cells; however, they suggested that these electrochemical responses might be related to the oxidation of guanine [45 48].
GloMax Discover is an integrated multimode detection plate reader system that offers scientists an integrated system to easily study cell signaling and metabolism, while monitoring cell viability, apoptosis, and cytotoxicity, or to study gene expression with a wide variety of reporter assays.
More suggestions(15)
monitor cell microenvironment
monitor cell uptake
monitor cell state
monitor cell respiration
monitor cell size
monitor cell responsiveness
monitor cell dissociation
monitor cell density
monitor cell fate
monitor cell cycle
monitor cell index
monitor cell biology
monitor cell phone
monitor cell repair
monitor cell membrane
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