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We used live cell imaging to assess cytotoxicity and spheroid morphology, microscopy to monitor cell migration and immunohistochemistry to assess extracellular matrix remodeling in response to direct and indirect LTP treatment.
There is a specific need to track these cells after transplantation, evaluate different methods of implantation, monitor cell migration within the body and quantify cell accumulation at the target site [2].
Here, cell proliferation was blocked by the addition of mitomycin C before cell stimulation to monitor cell migration exclusively.
The incubator was fastened to an inverted microscope (Live Cell Imaging CellR, Olympus, Japan) to monitor cell migration.
Confluent human SGC-7901 cell transfected with pEGFP-C1 or pEGFP-RUFY3 were subjected to a wound-healing assay to monitor cell migration.
CIM-plates are similar to a modified Boyden Chamber and can be used to monitor cell migration and invasion in real-time.
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RTCA (ACEA Biosciences) with CIM-plate (OMNI Life Science) monitored cell migration.
We next monitored cell migration and invasive behaviour in vitro.
xCELLigence Real-Time Cell Analyzer (RTCA; Roche Diagnostics, Switzerland) was used to monitor cell proliferation, migration and invasion independently in a label-free, real-time setting.
Sutton, E. J. et al. An optical imaging method to monitor stem cell migration in a model of immune-mediated arthritis.
For the validation of our platform, we demonstrate a coculture of epithelial cancer cells and endothelial cells and monitor the cell migration dynamics within the coculture.
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monitor cell uptake
monitor cell state
monitor cell respiration
monitor cell size
monitor whale migration
monitor cell responsiveness
monitor cell dissociation
monitor cell density
monitor cell fate
monitor cell cycle
monitor cell index
monitor smolt migration
monitor cell biology
monitor cell phone
monitor cell repair
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