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To monitor cell disruption, Trypan blue and a haemocytometer were used.
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In order to monitor the cell disruption process, two different methods of mechanical cellular breakage, sonication and porcelain mortar, were tested.
Our analysis indicated that the treatment was indeed just as efficient at cell disruption, as monitored by SDS-PAGE, as was sonication.
Cell disruption was monitored under the microscope.
Cells were thawed and disrupted using a high-pressure cell disruption system from Constant Systems Limited (United Kingdom).
In addition, TPEF and SHG images of hMSCs undergoing differentiation can be used to monitor cell morphology (TPEF) and the deposition of fibrous collagens (SHG) without the need for invasive processing or significant disruption of the culture conditions.
Zirmil-beating cell disruption.
Fig. 2 Cell disruption alternatives.
Biological cell disruption occurs by microbial degradation or enzymatic hydrolysis.
The effect of these factors on cell disruption was characterized.
Ultrasonication and microwave can be also used for cell disruption.
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