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We can monitor assembly of TBP, Brf2, Bdp1, and SNAPc on a U6 promoter by EMSAs [25], and so we checked whether addition of recombinant human Maf1 inhibited formation of either the four-factor complex or various subcomplexes on the U6 promoter.
By utilizing FRET signals from fluorescently labeled P/T DNA and PCNA to monitor protein-DNA interactions, we were able to observe both activities of RFC in real time and directly monitor assembly of the DNA pol holoenzyme.
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We used mass spectrometry (MS) to monitor assemblies formed between eukaryotic Hsp90/Hsp70/Hsp40, Hop, p23, and a client protein, a fragment of the glucocorticoid receptor (GR).
Although a high-throughput screening (HTS) assay that monitors assembly of 19S RPs with 20S cylinders has not been reported, an HTS assay for identifying inhibitors of Rpn11 and the Rpt enzymes was originally developed at Proteolix [ 58] and refined in my laboratory [ 59].
UV vis, fluorescence spectroscopy, XRD, SEM and AFM have been adopted to monitor the assembly process.
Piezoelectric quartz crystal impedance (PQCI) technique was employed to monitor the assembly of DNA in real time.
FT-IR, UV vis and PL spectroscopies were used to monitor the assembly process and also demonstrated that Mb was immobilized into the hybrid matrix without denaturation.
The UV Vis spectroscopy was used to monitor the assembly process, showing a progressive increase in immobilized bacteria amount upon deposited cycles.
A new technique invented at MIT can measure the relative positions of tiny particles as they flow through a fluidic channel, potentially offering an easy way to monitor the assembly of nanoparticles, or to study how mass is distributed within a cell.
Some checkpoints monitor proper assembly of the mitotic spindle and prevent abnormal segregation of chromosomes during the metaphase to anaphase transition [27].
We monitor PA assembly using a 1∶1 mixture of AF555-donor-and AF647-acceptor-labeled nicked-PA (nPA) monomers (50 nM each) and the ratio of the fluorescence emission intensities at 668 and 566 nm (F668/F566).
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