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Temperature, salinity and pH were determined at the moment of sample collection.
Temperature, salinity, and pH were determined at the moment of sample collection using a field thermometer, a hand-held refractometer (Leica) and pH strips.
In order to decrease the influence of the variables unknown at the moment of sample calculation, the sample size was increased to 80 subjects.
In addition, as detailed in Table 1, the proportion of patients under steroid treatment at the moment of sample collection during the hospitalization period was very similar in both groups of patients (41.6% for MV and 57.1% in NMV); in consequence, steroid treatment should affect similarly both groups in terms of modulation of the immune response.
To test whether the intensities of peaks that differed between cases and controls, also differed between cases that were more close to diagnosis, and cases that were less close to diagnosis at moment of sample collection, we performed independent sample T tests.
In order to avoid effects due to differences in the blood collection time, we compared mild and severe dengue patient groups using Mann-Whitney U Test, which showed no differences in the disease onset time at the moment of sample collection [see Additional file 1].
Similar(54)
Obtained data were normalized to internal standards and optical density of the culture at the moment of sampling (see Materials and Methods).
None of the staff carried pneumococci at the moment of sampling, making it less likely that animals had been infected recently.
Given that the generation interval of novel influenza A(H1N1) is probably in the range 2 4 days [5] [6], [16] [20], we believe that the moment of sampling of household contacts may have been close to optimal.
Although the moment of sampling of household contacts was probably close to ideal given that the generation interval of novel influenza A(H1N1) is approximately 2.2 3.2 days [5] [6], [16] [20], it is possible that some cases had been missed because sampling was performed too early or too late.
At the moment of sampling, sows of both herds were vaccinated with a EU-genotype attenuated vaccine (Porcilis® PRRS).
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CEO of Professional Science Editing for Scientists @ prosciediting.com