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After removing cell debris, CD8 chimeric molecules were precipitated with M2 anti-FLAG Ab-coupled sepharose (Sigma).
After pre-clearing of the lysate with Protein G-plus Agarose beads (Santa Cruz Biotechnology Inc., Santa Cruz, California, USA) the IgM molecules were precipitated using biotinylated anti-IgM antibodies (Jackson ImmunoResearch Inc).
PEGylated molecules were precipitated by pouring reaction solution into excess amounts of cold ether, followed by centrifugation and then three times of washes with cold ether.
Through the TPP method, proteins (other than gfp uv ), nucleic acids and other molecules were precipitated and separated from gfp uv.
When the Apaf-1-interacting molecules were precipitated in parallel experimental settings, full-length TCTP found to be associated with Apaf-1 in the TCTP-overexpressing untreated cells.
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As reported previously, the full-length lasp-2 molecule was precipitated with F-actin (Figure 2A, arrowhead).
As would be expected, and as was indeed seen by silver staining, other molecules also were precipitated along with PrPSc.
After 2 h of incubation, cell lysates were precipitated with α-Flag-agarose-beads and co-precipitated molecules were eluted with a Flag-peptide.
There are certain features of the undulating laminated films that would be difficult to explain if they were precipitated from fluids enriched in abiotic molecules.
To remove protein, to dissociate small molecules bound to protein or trapped in the precipitated protein matrix, and to recover chemically diverse metabolites, proteins were precipitated with methanol under vigorous shaking for 2 minutes followed by centrifugation.
Samples were precipitated by methanol/chloroform.
More suggestions(15)
elements were precipitated
molecules were generated
cores were precipitated
molecules were designed
molecules were compared
molecules were considered
molecules were collected
molecules were synthesized
molecules were chosen
molecules were expressed
molecules were selected
molecules were shown
molecules were involved
molecules were filtered
molecules were constrained
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