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To further corroborate the characteristics of OO extract-induced NET release, inhibition experiments of these classical NET-associated molecules were performed using specific inhibitors for NE (CMK), MPO (ABAH), and NADPH oxidase (DPI).

The optimized geometries of these molecules were performed using Hartree-Fork and Density Functional Theory (DFT-B3LYP) combined with the theory of atoms in molecules (AIM).

Active site ligand minimizations, that included, in turn, all docked molecules, were performed later.

Subsequently, these polyclonal cell lines that confirmed expression of Mamu-DR molecules were performed two rounds of limited dilution assays to obtain a stable monoclonal cell line that co-expresses DRα and DRβ chains simultaneously as our previous described.

BHPLC-MS analysis was performed with an agilent 1100 series high performance liquid chromatography (HPLC) connected to a micromass LCT TOF MS. The measurements for QS signal molecules were performed in "black 96 welled microtiter plates" (Nunc, black PolyBase, USA), using specific QS reporter strains.

To our knowledge all other studies in P. aeruginosa involving antibiotics as signal molecules were performed using the clinical isolates.

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Similar(47)

Characterization of the molecules was performed by physical and spectroscopic means and X-ray crystallographic analyses.

Design of these molecules was performed by utilizing a co-crystal structure for structure-based drug design.

The imaging of fluorescent DNA molecules was performed on a Nikon Ti-E inverted microscope equipped with a Nikon 100x oil-immersion objective (Nikon CFI Apo 100XW NIR) and an Andor iXon Ultra EMCCD camera.

The carboxyl moiety of the CMD was coupled to the aminated surface by EDC-NHS reagents, while CMD coupling through epoxysilane molecules was performed without any additional reagents.

Then, the cohesion between the amine cations, the inorganic parts and the free water molecules is performed via several types of hydrogen bonding forming interesting structural patterns.

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