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Next, two solutions containing the respective molecules were mixed at the desired molar ratio, followed by lyophilization of the mixed solution.
During the process, known as femtosecond spectroscopy, molecules were mixed together in a vacuum tube in which an ultrafast laser beamed two pulses.
The three DNA molecules were mixed with various gene carriers in solution to examine particle formation and morphology.
Briefly, serial four-fold dilutions of purified VHH molecules were mixed with 100 fluorescent focus-forming units (FFU) of human RV strain Wa (SbI, P[ 8]G1), followed by incubation for 1 h at 37°C.
In order to verify whether S100A8, S100A9 and TGFβ1 interact each other at a molecular level, these molecules were mixed at a 1 1 molar ratio in the presence of Ca2+, incubated overnight at room temperature and then analyzed by MALDI-TOF/MS.
siRNA, LNA or pre-miR molecules were mixed with 295 µl RPMI1640 (Lonza) and incubated for 10 minutes at room temperature.
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If there is aggregation of molecules in solution, then the current protocol could be modified to a dilution series with particular care taken to ensure molecules are mixed well and neither aggregate nor degrade in the process.
Indeed, explicit modelling of a situation in which two long polymer molecules are mixed in a small chamber, under conditions similar to those of replication, shows that an entropy-driven process will tend to segregate the molecules, in precisely the opposite direction to the standard mixing of Boltzman's gases (Jun & Mulder, 2006).
The reaction mixture, containing test sample in 160 µL dH2O or control vehicle (160 µL dH2O); 20 µL CETP assay buffer; 10 µL of donor molecule and 10 µL of acceptor molecule was mixed well.
The obtained molecule was mixed with 3 μl T4 DNA Ligase Buffer, 1 μl of 10 mM dNTPs and 0.5 μl of T4 Polymerase, and 0.5 μl of T4 PNK in a 2 ml microfuge tube.
The implicit representation is shown as follows: The modification information for the molecule is mixed together in the name of the molecule in tp:molecule.
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