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The molecules were displayed as skeletal formulae, drawn with the program ChemDoodle [68].
To make the ranking process easy, a simple web interface was prepared where all 40 molecules were displayed along with a menu which allowed selection of a score between 1 and 10.
Because these inhibitory molecules were displayed less than those presented by exhausted CD8+ T cells in chronic infection, it is probably speculated that exhausted CD4+ T cells were less similar to CD8+ T cells.
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Molecules are displayed in a 2D map projection based on chemical features or similarity.
The optimized geometries obtained by B3LYP/6-311++G** level of theory for the ground and excited states studied molecules are displayed in Figs. 10 and 11, respectively.
If 2D screening was performed, the two molecules are displayed side-by-side for easy visual comparison (Fig. 2); in case of the 3D screening, the two molecules are superimposed upon each other using the PyMOL pair fitting function, thus making it possible to visually compare the spatial arrangement of potentially important functional groups (Fig. 3).
The aligned molecules are displayed in multiple concentric circles, where each circle includes all molecules measured in the same comparative experiment, i.e., having the same EIN.
The disadvantage of the heat map is that all molecules are displayed on one axis so that it is difficult to see details of correlations for a single molecule if a large number of molecules are included.
At this juncture, there is no evidence that any domain of these molecules is displayed on the parasite surface, where it might respond to host or parasite derived ligands.
However, within the fibrils, the native AP molecules are displayed in a manner that is readily accessible to the substrate and catalyzes the phosphatase reaction with similar efficiency to the soluble form of the same enzyme construct.
We could demonstrate no surface expression of HLA-F by any cells at the implantation site by flow cytometry and all trophoblast populations were also negative by immunohistochemistry. Thus, at present, there is still only convincing evidence that three HLA-I molecules are displayed by human EVT cells: HLA-C, HLA-E and HLA-G (King et al., 2000; Apps et al., 2007, 2008).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com