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To explore the construction of bispecific molecules we also evaluated one other potential permissive site diametrically opposite the natural CDR-like loops, which was found to be tolerant of peptide insertion.
In order to have an independent source of H1 molecules, we also extracted H1 from SL2 cells (cell line of embryonic origin) and analyzed the C-terminal peptides.
Since mitochondrial cristae sequester the bulk of cytochrome c molecules, we also explored the impact of CHCHD10 mutations on apoptosis.
To extend our investigation beyond the CD94L-type class Ib molecules, we also included the rat RT1-M3 and mouse H2-M3 class Ib sequences.
Because HIFs promote the expression of Vegfa and other hypoxia-induced proangiogenic molecules, we also targeted the genes encoding HIF1α and HIF2α in myeloid cells with Lysm Cre.
Although our analysis focused on CDR3s, because these regions are known to interact with antigen peptides within the antigen-binding cleft of MHC molecules, we also analysed V and J usage (see Supplementary Fig. S2).
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To compare the binding mode of N-trifluoroacetyl derivative with that of the lead molecule, we also determined the structure of GPb NAG complex at a higher resolution (1.9 Å).
The Hemi-Indigo molecule [6, 7] was also studied because its properties have been shown to be similar to those of the Indigo molecule; we also thought it would be interesting to compare its properties with those of the rest of the investigated molecules.
Furthermore, as this group is not capable of completing the final phosphohydrolysis step which results in production of adenosine – another important signaling molecule, we also characterized tissue distribution of NT5E in bladder.
For the prediction of large number of molecules, we have also provided Python and R language based standalone package.
However, the polymerization with glutaraldehyde may also modify the tertiary and quaternary structure of the molecules as we also observed recognition of specific IgG antibodies to new regions or epitopes in the individual allergens.
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