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Synthesis of the selected molecules was done and In-vitro antimalarial activity was evaluated.
Then, electrochemical desorption of the organic molecules was done in 0.1 M KOH at an applied potential of −1.6 V vs. Ag/AgCl.
The geometrical optimization of the investigated molecules was done by Ab initio method at 6-31G** baset set for all atoms.
A classification of the OH⋯O hydrogen bonds (HBs) formed in all structures discriminating among the surface functional groups, solvation water molecules, and adsorbed molecules was done by the topological analysis of the electron density.
Their evaluation as drug-carrying molecules was done by measuring the molecular interactions with the anticancer agent, doxorubicin, and with the d-galactose-binding peanut lectin using an SPR optical biosensor.
Non-adherent cells were harvested and staining for cell-surface molecules was done as described above.
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Prior to the calculation using the Gaussian 9.0, optimizations of the inhibitor molecules are done by the same software.
All relaxation for PG and PtG sheets in the present and absence of HF and SO3 molecules were done using B3LYP density functional with split basis set (for all atom excluding Pt the basis set was 6-31G (d,p), for Pt atom the basis set was lanl2dz) as implemented in Gaussian 09 suite of program [33].
Synthesis of 5-cyano-N- 4- 4-methylpiperazine-1-yl -2- 4-methylpiperidin-1-yl phenyl)furan-2-carboxamide and 5-cyano-N- 4- 4-methylpiperazine-1-yl -2- 4-methylpiperidin-1-yl phenyl2-carboxamide molecules were done according to literature methods available with slight modifications for radiolabeling experiments3.
To illustrate how force measurements of molecules are done to obtain the parameters that define the mechanical properties of molecules, we use single molecule force studies of a protein with repeated units.
Size estimations of eccDNA molecules were done using a mixture of specifically designed markers including open circular molecules produced by Cre/lox recombination and nickase treatment of larger plasmid templates (see Methods for details).
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