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Methods are being developed and refined to generate sufficient numbers of cells able to be differentiated and retain their phenotype and perform the required biological functions, in the case of bone, the production of extracellular matrix, secretion of cytokines and other signaling molecules to enable mineralization to take place.
The FL-mapplet was also linked to the MQN-browser for fragrance molecules to enable MQN-nearest neighbour searches (see below).
In particular, at the beginning of drug discovery programs, activities are not available for a sufficient number of molecules to enable the generation of a good predictive model.
Molecule identifiers such as SMILES (Simplified Molecular-Input Line-Entry System [38]) or InChI (International Chemical Identifier [39]) strings can be associated to small molecules to enable direct molecule matching and similarity and substructure searches.
This is why this study had an additional step involving the addition of dATPs, using taq DNA polymerase which catalyses the non-template directed addition of an adenine residue to the 3′-end of both strands of DNA molecules to enable TA cloning in pGEM-T Easy vector.
While there have been several reports documenting the ability of a variety of adhesion molecules to enable osteoclastic adhesion to substrates, there has been only one previous comparison of the ability of adhesion molecules to activate actin ring formation.
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We have adopted a granularity model in which a DataCite persistent identifier (DOI) is created for each individual molecule to enable data discovery and data metrics at this level using DataCite tools.
In enhancer screens, putative enhancer regions drive the expression of a fluorescently labelled molecule to enable the dissection of regulatory regions of the genome.
In a typical DNA aptamer selection, a large single-stranded DNA library (up to 10 random sequences) is incubated with a target molecule to enable the binding reaction to occur.
It is not clear at present whether full-length Bid interacts with this protein or whether Bid phosphorylation changes the conformation of Bid molecule to enable its mitochondrial translocation.
The active domain and the Zn-binding domain are essential for catalytic activity: upon pro-domain cleavage a Zn2+-ion becomes available to coordinate with a hydrolytic water molecule to enable nucleophilic attack of a substrate, and the enzyme is functionally active [ 164].
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