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Successful caging of bioactive molecules requires the identification of key functional groups in appropriate molecules and the introduction of a suitable caging moiety.
The initial processing of Atg8 molecules requires the cysteine protease Atg4, which has multiple mammalian homologs.
Finally, the efficient tumor targeting of CD1d molecules requires the over-expression of a tumor antigen for which a high-affinity antibody scFv has been developed.
For instance, diffusion of small substrates through heme-dependent enzyme molecules requires the transient appearance of channels in the protein structure.
However, the release of AA from TAG molecules requires the reacylation of the DAG produced, a reaction involving the ATP-consuming activation of the fatty acid to acyl-CoA by long-chain ACSLs (acyl-CoA synthetases) [ 87].
Accommodating such large molecules requires the network to form an alternating sequence of catenane orientations positioned above and below the plane of the 2D sheet, with further long-range-ordering observed between the stacked arrays.
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Identification and development of these molecules require the use of newer techniques such as high-throughput screening of combinatorial compound libraries and the use of new bioinformatics tools.
However, communications via those molecules require the presence of the both sender and receiver species in the same liquid environments or in gel-like setting within a short distance to allow for diffusion of signaling molecules.
Consequently, the particles can slip between air molecules requiring the application of a Cunningham correction factor [ 22].
The process of association of one α-tubulin with one β-tubulin molecule requires the co-ordinated interaction of a series of tubulin-specific partners, designated tubulin cofactors (TBCs) A E [2] [5].
Because base hydrolysis of mycolactone (separating the lactone core and Northern chain) was shown to result in a molecule with ∼1000-fold ∼1000-foldtopathic activity towareduced9 fibroblasts (Mve-Obiang et al., 2003), it has been cytopathic the virulence of the molecule requires the Southern polyketide side chactivity
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