Hundreds of coordinated, multistep reactions, fueled by energy obtained from nutrients and/or solar energy, ultimately convert readily available materials into the molecules required for growth and maintenance.
Here, we describe a protocol for image-based whole-genome RNAi screening aimed at identifying molecules required for TGF-β signaling into the nucleus.
Genetic screens in the nematode C. elegans have led to the discovery of a number of molecules required for synapse patterning and assembly.
Forward genetic screens and antisense technology have helped to elucidate the signaling pathways and molecules required for inner ear development and function.
IRF4 regulated the expression of key molecules required for the aerobic glycolysis of effector T cells and was essential for the clonal expansion and maintenance of effector function of antigen-specific CD8+ T cells.
Recently, an increasing number of animal models derived from mutational screens, gene inactivation, and transgenic studies have identified specific molecules required for normal development of the cardiac valves, and critical molecular pathways are beginning to emerge.
Proteins are large molecules required for the structure, function, and regulation of the plant tissues.
Understanding the biochemical mechanism of a disease usually suggests the types of molecules required for new drugs.
Here, sensitivity is defined as the smallest quantity of input RNA molecules required for a gene to be confidently called.
can produce all the complex molecules required for biosynthesis (e.g. amino acids, vitamins and fatty acids), some need these complex molecules to be added artificially in a growth medium.
The information required to build all of the RNA and protein molecules required for life is stored within the order of the nucleotide bases in the DNA molecule.
