Sentence examples for molecules quantified by from inspiring English sources

For standard curve acquisition, eight serial 10-fold dilutions of linearized plasmid DNA containing the insert were prepared for each primer set, representing 30 to 3.0×108 double-stranded DNA molecules quantified by use of a BioPhotometer (Eppendorf, Hamburg, Germany).

To assess the fraction of immunoprecipitated (IP) DNA molecules among that of the total DNA (whole cell extract DNA, WCE) molecules, the number of IP and WCE molecules was quantified by real-time PCR using SYBR® Green I (BioWhittaker Molecular Applications, Rockland, ME, USA) and an iCycler Thermal Cycler (Bio-Rad Laboratories, HerCAles, CA, USA) as described previously.

In order to generate the similarity networks in the function of the similarity threshold ECFP fingerprints were generated for the molecules with a diameter of 4. Similarity of the molecules was quantified by the Tanimoto-similarity measure.

The number of molecules was quantified by fluorometry (Quant-iT PicoGreen dsDNA assay kit, Invitrogen, Carlsbad, CA).

The number of recovered cDNA molecules was quantified by limiting dilution PCR and compared to the HIV-1 RNA levels of the original plasma samples.

Following inactivation by various treatments, the residual DNase activity was measured by incubation of λ DNA in optimal conditions and the degradation of 73 bp and 153 bp λ DNA target molecules was quantified by qPCR (Table 3).

The diffusion of water molecules is quantified by measuring apparent diffusion coefficient (ADC) values.

For the direct Y-chromosome markers amplification, a mean of 366 ± 254 initial molecules was quantified by real-time PCR.

Then the bio information of the DNA molecules is quantified by comparing the photodiode signals measured at DNA spots and background regions on the HPV microarray.

The polarity of molecules can be quantified by a number called a dipole moment.

Channel blocking by the antibiotic molecules was also quantified by using a statistical analysis of the channel in its 'un-occupied' and 'occupied' (or blocked) states.