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The 400-nanometre version turned out an average of 190 protein molecules per vesicle.
Under our reconstitution conditions, we estimate that there are 50 60 β2AR molecules per vesicle.
In the model, ε is a scaling constant that subsumes various factors such as the number of synthase molecules per vesicle and the relationships between the unit of A i and the arbitrary units of E i and S i. γ is a scaling constant that subsumes factors such as the rate of synthesis per synthase molecule, the cell wall thickness, and conversion factors involving the units of various quantities.
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Therefore, we estimated the number of EGFR molecules per diffraction-limited, EEA1-negative vesicle that can be observed following 5 min of 10 ng/ml EGF stimulation.
Upon the fusion of vesicles, catecholamine molecules inside the vesicle were released onto the narrow interface between the cells and the SWNTs net and then interacted with them by π π stacking.
Diversity in the number of NPs per vesicle and number of vesicles per cell has recently been reported [54], and we also observe this variability in NP distribution among cells in cellular TEM images (Additional file 1: Figures S3, S5).
At 10 h, the cells exposed to PEGylated AuNPs in serum-free conditions were observed to have the highest number of AuNPs per vesicle, followed by decreasing numbers of AuNPs per vesicle as the serum concentration increased.
TEM images suggested that for PEGylated AuNPs, most intracellular vesicles contained only 1 to 3 NPs up to a maximum of 8 NPs per vesicle (within a cell slice) (Fig. 3, Additional file 1: Figure S2).
From the correlated data, we obtain probability distribution functions of vesicles per cell and nanoparticles per vesicle.
However, phospholipid enclosed vesicles released by both eukaryotes and prokaryotes into their environment remove harmful molecules by vesicle cargos.
In some contrast, rapid FP or MPER peptide-induced vesicle fusion has typically been observed at 500 1000 peptides per vesicle.
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