Sentence examples for molecules per bead from inspiring English sources
Beads incubated with excess kinesin moved smoothly along a microtubule for many micrometres, but beads carrying from 0.17-3 0.17-3n molecules per bead, moved, on average, only about 1.4 microns and then spontaneously released from the microtubule.
The calculated dilution factor was 5.25 and sequencing was performed as a full titration run without bead enrichment, i.e. the run was performed on a 40×75 plate, divided into 8 sectors (a titration run uses 4 of these sectors with different numbers of DNA molecules per bead i.e. 1,4,16, and 64 respectively).
Each cDNA library was titrated using 2, 4 and 8 cDNA molecules per bead.
For this project, a ratio of two DNA molecules per bead was used.
emPCR was set up using Titanium emPCR kits (Roche) at 0.25 copies of DNA molecules per bead (8.75 μl of 1 × 106 molecules/μl into 35 million beads).
We may estimate the number of bound DNA oligomers per bead using Poisson statistics: where μ is the average number of DNA molecules per bead and Pμ v) is the probability of finding ν molecules on one bead.
According to the 454 GS Junior protocol (Roche), an emulsion PCR was done on 2 molecules per beads.
Optical tweezers greatly improved the efficiency of the bead assay, particularly at the lowest kinesin concentrations (corresponding to approximately 1 molecule per bead).
We incubated two sets of beads with single-molecule per bead levels of biotinylated DNA templates encoding either tagged CpI or a negative control protein, chloramphenicol acetyltransferase (CAT).
Amplicon library pools were then amplified by emPCR at a 0.5 DNA molecule per bead ratio using the Lib-L emPCR Kit.
For the Illumina system, an appropriate number of clusters must be generated during library amplification, while for the 454/Roche system only a precisely quantified library will ensure a correct calibration of DNA/beads proportions that fosters the binding of a single DNA molecule per bead and the production of an "in-range" emulsion PCR yield.
