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1,000 molecules of target with 500, 1,000, and 5,000 molecules of competitor, respectively.
Ten identical PCR mixtures were prepared, as described above, each containing 100,000 molecules of target and competitor DNA.
In our experiment, we ensemble: 2,000 molecules of target with 750, 1,000, and 5,000 molecules of competitor, respectively.
One of these experiments (100,000 molecules of target with six competitor dilutions with three replicas of each point) was repeated three times in different days.
Both the mutant and wild type clone were then diluted out so that each contained 1 × 10 molecules of target sequence.
Each microdroplet thus acts as an individual PCR microsystem in which amplification from single molecules of target DNA occurs independently of the other droplets, with PCR products confined to their droplet of origin.
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One molecule of target miRNA can trigger cyclical cleavage of the chimeric probes to produce 2′-O-methyl RNA by DSN in the first round of amplification.
When molecules of the target gas are in contact with the porous surface, it can react.
Furthermore, LOR has its inherent advantages to be extended for screening molecules of novel target as well as its utility in data integration.
This method provided a linear assay over five to six orders of magnitude and reproducibly detected titres as low as 103 molecules of the target CVYV cDNA.
Our competitive PCR was able to detect at least 750 molecules of DNA target sequence in 50 μl of PCR.
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CEO of Professional Science Editing for Scientists @ prosciediting.com