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ScFv molecules lacking the C-terminal cysteine performed poorly in similar experiments.
In fact, the B-FN specific mAb BC1 reacts with an epitope localized on FNIII 7 that is accessible only when the ED-B is included in the FN molecule; on the contrary, another monoclonal antibody, IST-6, reacts only with FN molecules lacking the ED-B.
As shown in Figure 5, we also found that only Reaper molecules lacking the GH3 domain were unable to colocalize with MFN2.
Thus, KD1L17R-KT is homogeneous at the C-terminus, with ∼20% of the molecules lacking the first two amino acids at the N-terminus.
Hemoglobin molecules lacking the Bohr effect are able to retain O2 under conditions of elevated acidity caused by increased oxygen consumption [ 44].
Changes in the percentage of IgG molecules lacking the terminal galactose units in the oligosaccharide chains attached to CH2 regions have been investigated as a possible explanation for amelioration of RA during pregnancy.
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To make amorphous solid water, you need water vapor and a surface colder than about 140 kelvins--so cold that the molecules lack the energy to arrange themselves in a crystal lattice after sticking to the surface.
Using a combination of virus-specific and tag-specific primers during qPCR is thought to limit amplification of dsDNA from falsely-primed cDNAs, as these molecules lack the unique tag sequences added during the RT step.
Indeed, most modifications are present substoichiometrically (occupancy below 50%), meaning that roughly equal numbers of substrate molecules lack the modification.
The reason is that RNA molecules lack the first of our four properties: It is not much easier to discover a novel alternative phenotype than to discover this phenotype's genotype network.
Because pepsin-digested collagen molecules lack the telopeptide domains required for cross-linking and matrix stability, we also performed these experiments using acid-extracted collagen I, which retains intact telopeptide domains [ 36, 37].
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