Indeed the potential of pepstatin as a therapeutic molecule is hampered by its too low intracellular penetration.
However, the single-molecule detection is hampered by the inherent sensitivity of the method.
The large scale production of these molecules as demanded for commercial purposes is hampered by the heterogeneity of the solid-phase processes and by the low reactivity of high-molecular weight PEGs in solution.
In this way, Glu 224 is no longer capable of ionizing the 'catalytic' water molecule and, as a consequence, it is hampered from using the protons from this water to catalyze the final cleavage of the scissile bond.
Most frequently the use of bioactive molecules for the supplementation of food and beverages is hampered by stability limitations or inadequate intestinal absorption.
The development of controlled release forms is hampered by the low solubility of the molecule, which affects its absorption rate.
Research is hampered in other ways.
Public investment is hampered by excessive decentralization.
On the one hand, naked RNAi molecules are rapidly digested by nucleases in the serum after systemic administration; on the other hand, due to the negative surface charge of RNA, the entry into the cell cytoplasm is hampered.
Historically, heterologous production of small molecules has been hampered by the challenges in expressing complex functional pathways for the production of the non-native molecules.
