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The results offer a method to design and synthesize organic molecules for multiphoton materials.
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The data shown should assist in probe choice and experimental design for multiphoton microscopy studies.
The application of multiphoton microscopy to intravital imaging is discussed, along with a description of how to modify a standard brightfield or fluorescence microscope for multiphoton imaging of window chamber microvessels.
Multiphoton excitation fluorescence intensity (I MPEF) emitted by these molecules undergoing multiphoton excitation can be expressed as, I MPEF ≈ N.
Figure 3 shows the experimental apparatus for multiphoton microscopy.
We characterize long (up to 285 mm) gradient index (GRIN) lens endoscope systems for multiphoton imaging.
For multiphoton measurements, a tunable wavelength Chameleon laser (Coherent) was applied.
The ability to deliver ultrashort pulses to the sample is critically important for multiphoton imaging.
The biopsy specimen was placed in the Glass Bottom Dish (MatTek, coverglass: 0.085-0.13 mm) for multiphoton imaging.
A key requirement for multiphoton microscopy is the need to fluorescently label cells, sub-cellular compartments or proteins of interest.
Multiphoton FLIM-FRET imaging was carried out on a Nikon Eclipse TE2000-U inverted microscope with an Olympus long working distance 20 × 0.95 numerical aperture water immersion lens using a scan head specifically designed for multiphoton excitation.
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