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These markers may serve as target molecules for aptamer selection.
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The first assumption is reasonable considering that aptamer molecules for one specific amino acid might have descended from a common ancestor RNA by duplication.
Recently, a new class of molecules termed aptamer has emerged as excellent molecular probes for cancer diagnosis and targeting therapy [ 14, 15].
Detection of protein using aptamer has been based on recognition between target molecules and aptamer in a 1 1 stoichiometric ratio.
Together, these data show that aptamer arrays are a powerful tool for aptamer screening.
Nevertheless, it seems likely that the optimum spacer length for aptamer microarrays will need to be independently optimized for each aptamer and for each labeling dye.
Moreover, the chemical and physical conditions for aptamer selection can be adapted to the real environment in which the aptamer will finally be applied.
A novel small molecule probe, aptamer beacon (AB), was introduced for adenosine (Ade) recognition and quantitative analysis.
The binding of small molecules to an aptamer is highly reversible, so it is possible to regenerate the sensor.
Incorporation of a small molecule-sensing aptamer domain into the ribozyme renders its activity ligand-dependent.
In the presence of the target molecule, the aptamer changes its structure to bind the target molecule.
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