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There were no significant increases in any of the seven cholesterol precursor molecules assessed in any of the brain regions (Fig. 10).
Besides, the levels of ErbBs, ligands and associated molecules, assessed in a microarray study, remain normal in embryonic p35 KOs (Supplementary Table 2), as well as cell proliferation and cell death in the embryonic forebrain in both the p35 and ErbB4HER4 heart KOs (Supplementary Fig. 5).
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The selection of a trial (school) and test (for prediction) set of molecules from a pool of available molecules does not necessarily set the domain of applicability, but once such a domain is available or defined, certain molecules are assessed in the trial and test series.
These calculations relied solely on the total number of miR-122 molecules assessed by qRT PCR and do not indicate the number of available miR-122 molecules in a cell containing other target sites, especially miR-122 decoy transcripts that primarily act to sequester miRNAs.
The resulting molecules were assessed in vitro for their 5HT1A affinity and half-life in a heterologously expressed human CYP3A4 assay.
In vitro, expression of prothrombotic molecules was assessed in human endothelial cells by real-time PCR and flow cytometry.
Relative productivity of variants and parental molecules was assessed in suspension cultures of CHOK1SV cells, using small-scale transient transfections in 96 well plates.
Because itraconazole induces changes in transcript levels in the ergosterol pathway and disturbs the total intracellular ergosterol content [ 15], the localization of ergosterol molecules was assessed in Paracoccidioides Pb01 and Pb18 yeast cells.
Sequence coverage across the molecule was assessed in terms of a) redundant and bi-directional coverage, b) the degree of additional manual re-processing that would be required to develop complete replicate coverage, and c) the correlation between sequence coverage and sequence distance from the revised Cambridge Reference Sequence (rCRS) [ 22].
BEAMing relies on single-molecule PCR at a massively parallel scale that millions of individual DNA molecules can be assessed in this fashion with standard laboratory equipment, similar to next-generation DNA sequencing technologies [ 44, 45].
Gene expression profiles for a large number of other antimicrobial immune molecules were also assessed in the equine uterus in our study (Fig. 2c).
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