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Alternatively, cells were stained with OX6 antibody (recognizing MHC Class II molecules) as previously described [10].
HUVECs were transfected with pre-miR or LNA molecules, as previously described, for 48 h.
In fact modulation of the pharmacophore elements present in bile acids could provide high affinity binding molecules, as previously testified by the development of TGR5 and FXR agonists [34].
It is likely that α1-PDX can block the processing of other proteins implicated in the invasive potential of tumor cells, such as growth factors and adhesion molecules, as previously reported in vitro and in vivo models [22], [23], [25], [38].
Before experimental use, each preparation was extensively characterised for potential contamination by other cell types and for expression of tight junction-associated molecules as previously described [ 8].
A hallmark of many dependence receptors involves receptor modification through intracellular cleavage by caspase or caspase-like molecules, as previously shown with EphA4.
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One could postulate that such an effect may indicate that the anesthetic binding site in LyTASK could accommodate two halothane molecules, as has been previously shown on anesthetic binding to cholesterol oxidase and firefly luciferase.
These studies revealed a number of previously unobserved features of ArcLight's behavior, including millisecond-scale fluorescence fluctuations in single molecules as well as a previously unreported delay prior to macroscopic fluorescence onset.
The formation of amine byproducts in the reactions involving 3– 5 could have resulted from abstraction of a proton from adventitious water or a solvent molecule, as previously reported by Hartwig[ 30] and Ribas[ 62].
Hipk2-null mice were generated through homologous recombination by eliminating the exon 3 coding for the entire catalytic domain at the N-terminus of the molecule, as previously reported.
We applied kDNA depletion methods to minimize the presence of this type of molecule, as previously described [ 58], which result in less than about 5% of remaining kDNA in the sample.
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