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It was only a brief experiment, but other groups have continued to extend the work to show that the molecules are functional and safe in the primate brain, which is more similar to the human brain.
There are lingering questions, however, about whether most of these molecules are functional or just spurious byproducts of transcription.
Recent studies focused on these neglected molecules have proven that these molecules are functional and of great potential.
In the particular case of intracellular proteins following exocytosis or recycling pathways, a supplementary level of complexity has to be taken into account which is linked to the knowledge of the cellular confinement where those molecules are functional or delivered.
Since chimeric CD44/5T4TM molecules are functional in facilitating CXCR4 membrane expression and chemotactic response one might conclude that the 5T4 specific antibodies are unlikely to influence the initial direct binding of CXCL12.
When all the molecules are functional, the state of the output molecule caspase3 can be determined using the equations (Table 1), for eight different set of inputs.
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The results showed that the immobilised scFvCLcys molecules were functional and specific binding curves (with response relative to the concentration of virus antigen) were obtained over more than 25 cycles of binding and dissociation.
Surprisingly, these molecules were functional and could transduce signals in recipient cells.
Interestingly, the cortactin molecule was functional, since it mediated additional targeting of the EGFP-tagged fusion protein to its physiological localization in lamellipodia and microspikes (Figure 5A), and was expressed as a full-length protein as confirmed by western blotting (Figure S5).
In this study, to determine the role of p53 plays in PEG-IFN/5-FU combination treatment efficacy, we selected the HepG2 cell line, as its p53 molecule is functional and does not bear mutations.
With the denomination of 'non-coding RNA' we identify those RNA molecules which are functional but that are not translated into proteins.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com