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We also stress the importance of biomarkers for medical surveillance of populations at risk and propose the use of biosensors as sensitive resources to detect undesirable effects of new molecules and environmental pollutants.
A large number of cytochrome P450s are responsive to hormones, signaling molecules and environmental stresses (for review: [ 91]).
To quantitatively assess the dose-response behavior of endogenous molecules and environmental chemicals, benchmark dose (BMD) methods have been employed to estimate reference doses [ 11- 13].
The vast majority of strategies to expand HSCs in vitro have focused on regulation of stem cell renewal and survival of HSCs mediated by intrinsic factors (transcription factors and signaling molecules) and environmental cues (cytokines, chemokines, and adhesion molecules) [ 12].
Nalp3 is a 'general sensor' for danger signals, representing an important caspase-1-containing inflammasomes and is activated by various pathogens [ 18], and damage-associated molecules and environmental irritants [ 40].
This increases cellular adhesiveness, which facilitates multicellular aggregates that are more resistant than planktonic cells to an assortment of antibacterial molecules and environmental stresses (Colvin et al., 2011; Billings et al., 2013).
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We and others have shown that zebrafish responded biologically to chemicals, such as small molecules, drugs and environmental toxicants, in a similar manner as mammals [ 21- 24].
Spectroscopy has proven to be crucial for understanding the structures and kinetics of myriad molecules and their environmental interactions.
This ensures that also conformational changes of the aptamers induced by the influence of the target molecule and other environmental impacts while binding are abstractly regarded in the libraries creation process.
Cells in different locations of 3D tissues are physiologically different, because they are exposed to different concentrations of oxygen, nutrients, and signaling molecules, and to other environmental factors (temperature, mechanical stress, etc).
Although herbicides are likely to play an important role, the evolution of herbicide resistance, declining rates of commercial release of new herbicide molecules, and concerns about environmental health and weed species shifts will militate against sole reliance on herbicides.
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