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Although a quantum chemistry simulation with chemical accuracy of the ground state energy of a large molecule would require many gates [43], including bosonic degrees of freedom can significantly reduce this number.
Activation of a water molecule would require its deprotonation, but the active site has no bases or metals that would deprotonate water.
Antibody binding to a high-entropy molecule would require a greater activation energy than binding to a lower-entropy molecule and may thereby introduce structural or functional constraints in either the FWR or CDRs.
We suggest that an indolocarbazole-sized molecule would require an opening with a radius of at least 4.5 Å; however, the pathway out of the reduced-RebC structure from where a substrate would be positioned does not become 4.5 Å wide until just before the surface of the enzyme.
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Yet less than 40% of molecules are correct at 200 bases; longer molecules would require higher efficiencies.
Considering the thickness of the membrane as well as the multiple steps, unfolding one mole of β-barrel MP molecules would require ~103 kJ of energy (equivalent to 400 RT or energy from hydrolyzing 20 30 moles of ATP).
A hundred EGFR molecules would require ∼12 clathrin-coated vesicles for delivery to endosomes (see 'Materials and methods').
Therefore, a proposed model suggested that, to produce βH crystals, heme dimers are formed by means of reciprocal iron-carboxylate linkage between heme molecules, which would require the displacement of the axial water molecule bound to the porphyrin [24], [27].
The closest relevant entry we found was "Cell adhesion molecules (CAMs)" [KEGG:hsa04514], but linking "the CD2 pathway" to "Cell adhesion molecules (CAMs)" would require significant background knowledge.
We note that the evaluation of unsaturation index for only TAG molecules by GC analysis would require fractionation of TAG class lipids prior to the chemical derivatization.
Since one DT molecule catalyses the ADP-ribosylation of 2000 EF-2 molecules/min in vitro [38], and since one cell contains about 106 EF-2 molecules [39], it can be roughly estimated that once a single DT molecule has entered the cytoplasm it would require about 30 h to inactivate all the EF-2 molecules in a cell [40].
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