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Each molecule was tested in triplicate.
The expression of each molecule was tested in cancer and normal-matched adjacent tissues.
Each molecule was tested at concentrations between 0.03 and 100 μM.
The antiviral effect of each molecule was tested alone or in combination to determine the CI.
Each molecule was tested at 10 μM on two separate occasions.
Being a major compound, the molecule was tested for possible relaxant activity on spontaneous rabbits' jejunal preparations.
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If a more potent drug is found, the molecule is tested in cells and animals to mimic the drug's behavior in humans.
It involves antibodies on a surface reacting with the molecule being tested for, and so activating a dye.
At the same time, the Amgen team looked at 53 "landmark" cancer-related preclinical studies (where a potential drug molecule is tested to determine correct dosage and whether it is toxic, usually in animal models).
Recombinant fusion proteins corresponding to the extracellular domains (inclusive of the PD-1Δex3 variant) of PD-1 molecule were tested in T-cell proliferation assays using RA-derived peripheral blood mononuclear cells (PBMCs).
To narrow down the binding site, binding of KatA to a range of truncated Vn molecules was tested by ELISA (Fig. 4b).
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CEO of Professional Science Editing for Scientists @ prosciediting.com