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The solution was incubated at RT for 2 h, and unreacted small molecule was removed by washing the solution three times with 10 mM citrate buffer (pH 3) using an Amicon Ultra-0.5 centrifugal filter unit, 3 kDa.
The enzyme was prepared for docking studies where: (1) Ligand molecule was removed from the enzyme active site.
The enzyme was prepared for docking studies where: (i) Ligand molecule was removed from the enzyme active site.
In the same study, similar results were observed when the starch molecule was removed and the samples were diluted with either a balanced electrolyte solution or 0.9% saline.
The amiR precursor molecule was removed from the pCR2.1 vector by using the SpeI and MluI restriction sites and ligated into the appropriate sites of pRed vectors.
Src– and Hck ligand substrate complexes were generated by incubation of SFKS245C with 6 or 7. Excess small molecule was removed through Ni-affinity resin binding followed by desalting.
Similar(53)
In vertebrates the all-trans retinal, produced when a photon isomerizes the 11-cis retinal of a rhodopsin molecule, is removed from the rod or cone.
In the decay step, every molecule is removed with a probability d = 0.03.
In this transformation, another sugar molecule is removed from Rh2 (see Discussion and Figure 1).
CBZ was superimposed onto the bound structure of metyrapone, and water molecules, ions, and the metyrapone molecule were removed.
If modeling of the suspected guest cannot be completed, then it is advisable that the incomplete molecule be removed.
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