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Pulses of red illumination are indicated as red shaded portions in the single molecule time trace.
(B ) Histogram of the all single molecule time traces exhibiting FRET displays a broad distribution.
(B ) Single molecule time traces showing periodic DNA patrolling by monomeric Pif1.
(C ) Histogram of all single molecule time traces exhibiting FRET displays a broad distribution.
(A ) Single molecule time traces of Pif1 dynamics on DD31 at two different protein concentrations.
(B ) Single molecule time traces of Cy3 (green), Cy5 (red) and Cy7 (black) intensities (top panel).
Similar(33)
One molecule timed out and did not generate a fingerprint.
We also would like to point out that the signal to noise ratio in FCS is proportional to the fluorescence count rate per molecule times the square root of the total experiment acquisition time [ 16].
A reversible local folding step in which a duplex docks and undocks from the ribozyme core was observed directly in single-molecule time trajectories, allowing the determination of the rate constants and characterization of the transition state.
Single-molecule time traces showed homogeneous FRET values, with little dynamics under all tested conditions.
Single-molecule time traces for P2−P3 were examined in more detail.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com