These findings will be helpful in experimental design, artifact identification, and data analysis for single molecule studies of various proteins using the mechanical unfolding method.
Single molecule studies of E. coli proteins have demonstrated that in steady-state they follow a gamma distribution [10], while protein bursts follow a geometric distribution [11].
We have found that the apparent distance between putative origins is about 3 5 kb in all cancer cells lines tested, a much shorter distance than that deduced from single molecule studies of DNA replication [25], [26].
Single-molecule study of oxidative enzymatic deconstruction of nanocrystalline cellulose by C1 and C4 oxidizing types of LPMO (NcLPMO9F, NcLPMO9C) was performed.
Davenport, R., Wuite, G., Landick, R. & Bustamante, C. Single-molecule study of transcriptional pausing and arrest by E. coli RNA polymerase.
A different single-molecule study of pausing at multiple sites did not detect backtracking at sequences that induced RNAP pausing.
In this manuscript, the authors performed a single-molecule study of ribosome translocation using high-precision optical tweezers.
Based on a previous single-molecule study of the Rev-RRE system, the timescale for each Rev binding step is ∼2 5 s (Pond et al., 2009).
A recent single-molecule study of transcription through modified nucleosomes showed that removal or mock acetylation of the histone tails has a modest effect on overall transcription elongation.
