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The Oxford Nanopore Technologies ONTT) MinION is one of the latest of a new generation of single molecule sequencing technologies.
Though some single molecule sequencing technologies have been developed, they are still too expensive and labor-consuming.
Of particular interest, in relation to this problem, are the single molecule sequencing technologies, like that recently brought to market by Pacific Biosciences of California (http://www.pacificbiosciences.com).
The new generation of single molecule sequencing technologies, such as the RS platform from Pacific Biosciences with longer reads and different error profiles, have brought their own QC challenges.
With the availability of the so-called Next Generation Sequencing (NGS), relatively cheap high-throughput short molecule sequencing technologies such as Illumina GA and ABI SOLiD, and medium length sequencing technologies such as Roche 454 is giving non-specialist laboratories the ability to sequence large genomes.
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The first human genome sequenced using single molecule sequencing technology.
In August, Stephen Quake, a bioengineer at Stanford (and TR35 winner in 2002) announced he had sequenced the first human genome using single molecule sequencing technology–his own.
Stephen Quake, a bioengineer at Stanford University, has sequenced his genome the first human genome generated using single molecule sequencing technology, according to results published in the current issue of Nature Biotechnology.
One technical challenge of producing the longer segments was the error rate of 10 to 15percentt in the single molecule sequencing technology used.
Patrice Milos, vice-president and chief scientific officer, says the ARRA funding has been "a pivotal grant that has enabled us to hire and retain key employees and to continue development work" on the 7-year-old company's single molecule sequencing technology.
SMRT DNA sequencing is a single molecule sequencing technology, whereby the continuous incorporation of phospholinked nucleotides by a DNA polymerase is detected as fluorescent pulses.
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