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The amounts of dye incorporated cDNA were measured by spectrophotometer using the acceptability cutoffs of > 200 pmol of dye incorporation and a nucleotide to dye molecule ratio of > 50 [ 91].
To determine the TTR stabilization effect of SOM0226 and tafamidis, the small molecules (dissolved in DMSO) were first preincubated for 30 min at room temperature with the desired TTR variants (8 μM) at a TTR/small molecule ratio of 1 2.
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From the mean values, the molecule ratios of prostein to HPRT transcripts were calculated.
Each determination was carried out twice for each cDNA sample as independent PCR runs and molecule ratios of SOX2 to β-actin transcripts were calculated from the mean values.
However, low plasmid concentrations occasionally gave abnormally large numbers of molecules (ratio of observed to expected molecules above 1.5) with the fast mixes (Roche and QuantiFast).
Mid-regional pro-adrenomedullin (MR-proADM) is a fragment of 48 amino acids which splits from the final proADM molecule in a ratio of 1 1 with ADM.
A "tricreatine citrate" is actually a complex of creatine citrate with two additional creatine moieties, resulting in a molecule with a ratio of creatine to citrate of 3 1.
In order to have a more convenient term for sulfotransferase assay, we further defined relative mobility (M') of a small molecule as the ratio of its mobility to that of PAPS (M p ). (2) Since the charge and mass of PAPS (q p and m p respectively) under defined conditions are constant, M' of a small molecule is only dependent on its own q/m ratio.
The higher the signal of either SSP or PPP the higher is the ordering of the molecules while the ratio of SSP/PPP determines the average orientation of the interfacial molecules.
For ZSM-5, transition from Knudsen regime to configurational regime may occur for roughly spherical molecules when the ratio of molecular diameter to channel diameter, λ, is greater than approximately 0.6 0.8.
An input DNA molecule-to-bead ratio of 1.5 was used.
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